Dr. Chanakan Tongsook

dummyBiochemistry

Email: tongsook.c@gmail.com, tongsook_c@su.ac.th

Room: 223 Science Building 3

Tel.: 034-109686 ext. 207313

Website:-

Education:

Ph.D. (Biochemistry) Graz University of Technology, Austria

M.Sc. (Biochemistry) Mahidol University, Thailand

B.Sc. (Chemistry) Silpakorn University, Thailand

Research Interest:

Mechanistic and molecular enzymology, enzyme kinetics, biocatalytic synthesis, and protein biochemistry

Publications:

  • Tongsook, C., Boonkumkrong, R., Thepthit, Y. (2022). “Enhancement of recombinant N,N-8-amino-8-demethyl-D-riboflavin dimethyltransferase (RosA) protein expression in E. coli for production of antibiotics and flavin-derivatives.” Pure and Applied Chemistry International Conference 2022 Proceeding,  June30 -July 1,2022. 715-721. King Mongkut's Institute of Technology Ladkrabang, Bangkok.

  • Pitsawong, W., Chenprakhon, P., Dhammaraj, T., Medhanavyn, D., Sucharitakul, J. and Tongsook, C., van Berkel, WJH., Chaiyen, P., and Miller, AF. (2020). “Tuning of pKa values activates substrates in flavin-dependent aromatic hydroxylases.” J Biol Chem., 295(12): 3965-3981. (SCOPUS impact factor 4.238 in 2019)

  • Chenprakhon, P., P. Pimviriyakul, Tongsook, , Chaiyen, P. (2020). Phenolic hydroxylases. The Enzymes, Elsevier. 47: 283-326.

  • Pornsuwan, S., Maenpuen, S., Kamutira, P., Watthaisong, P., Thotsaporn, K., Tongsook, C., Juttulapa, M., Nijvipakul, S., Chaiyen, P. (2017). “3,4-Dihydroxyphenylacetate 2,3-dioxygenase from Pseudomonas aeruginosa: An Fe(II)-containing enzyme with fast turnover.” PLoS One, 12(2): e0171135.

  • Tongsook, C., Niederhauser, J., Kronegger, E., Straganz, G., Macheroux, P. (2017). “Leucine 208 in human histamine N-methyltransferase emerges as a hotspot for protein stability rationalizing the role of the L208P variant in intellectual disability.” Biochimica et Biophysica Acta, 1863(1): 188-199.

  • Dhammaraj, T., Pinthong, C., Visitsatthawong, S., Tongsook, C., Surawatanawong, P., Chaiyen, P. (2016). “A single-site mutation at Ser146 expands the reactivity of the oxygenase component of p-hydroxyphenylacetate 3-hydroxylase.” ACS Chemical Biology, 11(10): 2889-2896.

  • Tongsook, C., Uhl, MK., Jankowitsch, F., Mack, M., Gruber, K., Macheroux, P. (2016). “Structural and kinetic studies on RosA, the enzyme catalysing the methylation of 8-demethyl-8-amino-d-riboflavin to the antibiotic roseoflavin.” FEBS J, 283: 1531-1549.

  • Heidari, A., Tongsook, C., Najafipour, R., Musante, L., Vasli, N., Garshasbi, M., Hu, H., Mittal, K., McNaughton, AJ., Sritharan, K., Hudson, M., Stehr, H., Talebi, S., Moradi, M., Darvish, H., Arshad Rafiq, M., Mozhdehipanah, H., Rashidinejad, A., Samiei, S., Ghadami, M., Windpassinger, C., Gillessen-Kaesbach, G., Tzschach, A., Ahmed, I., Mikhailov, A., Stavropoulos, DJ., Carter, MT., Keshavarz, S., Ayub, M., Najmabadi, H., Liu, X., Ropers, HH., Macheroux, P., Vincent, JB. (2015). “Mutations in the histamine N-methyltransferase gene, HNMT, are associated with nonsyndromic autosomal recessive intellectual disability.” Hum Mol Genet, 24: 5697-5710.

  • Bernkopf, M., Webersinke, G., Tongsook, C., Koyani, CN., Rafiq, MA., Ayaz, M., Müller, D., Enzinger, C., Aslam, M., Naeem, F., Schmidt, K., Gruber, K., Speicher, MR., Malle, E., Macheroux, P., Ayub, M., Vincent, JB., Windpassinger, C., Duba, HC. (2014). “Disruption of the methyltransferase-like 23 gene METTL23 causes mild autosomal recessive intellectual disability.” Hum Mol Genet,: 4015-4023.

  • Sucharitakul, J., Tongsook, C., Pakotiprapha, D., van Berkel WJ, Chaiyen, P. (2013). “The reaction kinetics of 3-hydroxybenzoate 6-hydroxylase from Rhodococcus jostii RHA1 provide an understanding of the para-hydroxylation enzyme catalytic cycle.” J Biol Chem, 288: 35210-35221.